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Updated: Sep 14, 2025

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
Published on: October 9, 2020
Indirect Zero-Field Nuclear Magnetic Resonance Spectroscopy
Kai Buckenmaier1, Richard Neumann1, Friedemann Bullinger1
1High-Field Magnetic Resonance Center, Max Planck Institute for Biological Cybernetics, Tübingen 72076, Germany.
Abstract:
This study develops the two-field correlation spectroscopy (COSY) in zero to ultralow field (ZULF) liquid state nuclear magnetic resonance (NMR). We demonstrated the successful integration of signal amplification by reversible exchange (SABRE) hyperpolarization with two-dimensional (2D) NMR spectroscopy, enabling the detection of ZULF COSY spectra with increased sensitivity. Field cycling allowed the acquisition of two-field COSY spectra at varying magnetic field strengths, including zero-field conditions. This enabled insight into both J-coupling and Zeeman-dominated regimes, benefiting from ultralow field observation sensitivity and mitigating the low-frequency noise by conducting readout at higher fields (>5 μT). Our study explores the effects of polarization transfer, apodization techniques, and the potential for further application of ZULF NMR in chemical analysis exemplified for three X-nuclei and three corresponding molecules: [1-13C]pyruvate, [15N]acetonitrile, and [3-19F]pyridine. These findings pave the way for more sensitive and cost-effective NMR spectroscopy in low-field regimes.
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