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Droplet-Based Measurements of DNA-Templated Nanoclusters-Towards Point-of-Care Applications
Jonas Kluitmann1, Stefano Di Fiore2, Greta Nölke2
1Institute of Sensor and Actuator Technology, Coburg University of Applied Sciences and Arts, Am Hofbräuhaus 1b, 96450 Coburg, Germany.
Biosensors
|July 25, 2025
Summary
Fluorescent DNA-silver nanoclusters show potential as sensors for point-of-care testing (PoCT). A microfluidic platform demonstrated their sensitivity to environmental changes, highlighting feasibility and challenges for biological applications.
Area of Science:
- Nanoscience
- Biotechnology
- Analytical Chemistry
Background:
- Fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) are emerging as novel nanomaterials.
- Their application as sensors, particularly for point-of-care testing (PoCT), requires thorough investigation of their stability and response in relevant environments.
Purpose of the Study:
- To assess the fundamental usability of DNA-AgNCs as sensors for PoCT.
- To evaluate the response of DNA-AgNCs to variations in pH and sodium chloride concentration within a microfluidic platform.
Main Methods:
- Development of a microfluidic platform for generating droplets with DNA-AgNCs in controlled chemical conditions.
- Fluorescent readout of droplets at two emission wavelengths.
- Acquisition of response data for two DNA-AgNCs to pH and NaCl concentration variations.
Main Results:
- The microfluidic setup detected DNA-AgNCs at concentrations below 100 nM.
- DNA-AgNCs maintained fluorescence across physiologically relevant chloride concentrations (up to 150 mM).
- Significant, complex fluorescence intensity changes were observed upon pH shifts, with distinct responses between different DNA-AgNC models.
Conclusions:
- DNA-AgNCs are feasible as sensors in a simple microfluidic setup for PoCT applications.
- The study highlights the potential of DNA-AgNCs as building blocks for biohybrid nanosensors.
- Challenges remain in adapting DNA-AgNCs for reliable use in complex biological matrices due to their environment-specific responses.
