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Related Experiment Video

Updated: Sep 13, 2025

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Multilayered Tissue Assemblies Through Tuneable Biodegradable Polyhydroxyalkanoate Polymer (Mesh)-Reinforced

Vasilena E Getova1,2, Alex Pascual1, Rene Dijkstra3

  • 1Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.

Gels (Basel, Switzerland)
|July 25, 2025
PubMed
Summary

Researchers developed a novel multi-layer cell construct using decellularised extracellular matrix hydrogels and biodegradable polymer meshes for regenerative medicine. This innovation supports diverse cell types and offers tuneable degradation for personalized tissue implants and therapies.

Keywords:
3D printingP34HBPHBVbiodegradationextracellular matrixhydrogelmelt electrowriting

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Growing demand for regenerative medicine therapies necessitates innovative solutions for in vitro cell constructs.
  • Existing methods may lack the necessary mechanical support and biological cues for complex tissue regeneration.

Purpose of the Study:

  • To develop a novel multi-layer cell construct integrating decellularised extracellular matrix (dECM) hydrogels and biodegradable polymer meshes.
  • To create a customizable and tuneable scaffold for supporting multiple cell types and applications in regenerative medicine.

Main Methods:

  • Fabrication of 3D-printed biodegradable polymer meshes using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB).
  • Integration of organ-derived dECM hydrogels with the polymer meshes.
  • Characterization of construct properties, including mechanical stability, pore size, fibre arrangement, and degradation kinetics after precoating with PHB depolymerase (PhaZ).
  • In vitro assembly of a three-layered tissue construct (epidermal, vascular, subcutaneous).

Main Results:

  • The combined construct integrates the mechanical stability of PHBV+P34HB with the biological properties of dECM hydrogels, supporting cell survival and function.
  • Constructs demonstrated tuneable degradation, with complete polymer degradation in 3-5 days, delayed to 10 days with hydrogel incorporation.
  • A proof-of-concept three-layered tissue construct representing different tissue types was successfully created in vitro.
  • Customization of fibre arrangement and pore sizes allows for personalized design.

Conclusions:

  • The novel dECM-polymer composite construct provides a versatile platform for multi-layer tissue engineering.
  • Tuneable degradation and customizable design make it suitable for various regenerative medicine applications.
  • Potential applications include advanced wound healing solutions, personalized drug delivery, and tissue implants.