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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Synthesis of a Light-Up Probe with a Trioxazole Skeleton for Tracking G-Quadruplex Dynamic Behavior.

Haruki Fujita1, Naruyuki Watatani1, Shogo Sasaki2

  • 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.

Analytical Chemistry
|July 25, 2025
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Summary

Researchers developed a novel fluorescent probe, TO-2,3VN (2f), to track guanine-quadruplex (G4) dynamics in living cells. This probe enables real-time visualization of G4 folding and unfolding, aiding in understanding their biological roles.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Chemical Biology

Background:

  • Guanine-quadruplexes (G4) are noncanonical nucleic acid structures vital for biological processes.
  • Tracking dynamic G4 folding and unfolding in living cells presents a significant challenge.
  • Developing specific probes is crucial for investigating G4 functions in vivo.

Purpose of the Study:

  • To synthesize and characterize novel fluorescent probes for real-time monitoring of G4 dynamics.
  • To develop a light-up probe capable of visualizing G4 formation and dissociation in living systems.
  • To assess the probe's specificity and utility in studying RNA G4 dynamics under cellular stress.

Main Methods:

  • Synthesis of trioxazole-based fluorescent probes incorporating vinyl naphthyl (VN) groups.
  • Evaluation of probe interaction with G4 structures, including stabilizing ability and light-up properties.
  • In vitro and in vivo experiments using the probe TO-2,3VN (2f) to visualize G4 dynamics in HeLa cells.

Main Results:

  • The probe TO-2,3VN (2f) selectively interacted with G4 structures with minimal interference in folding.
  • Remarkable light-up properties allowed visualization of G4 folding/unfolding in vitro.
  • TO-2,3VN (2f) specifically detected RNA G4 dynamics in living HeLa cells during starvation stress.

Conclusions:

  • TO-2,3VN (2f) is a promising fluorescent probe for real-time visualization of G4 dynamics in living cells.
  • The probe facilitates the study of dynamic G4 processes, particularly RNA G4s.
  • This tool can advance the investigation into the biological significance of G4 structures.