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Published on: December 2, 2022
Oyster (Crassostrea hongkongensis) protein isolates: Influence of extraction pH on physicochemical, conformational
Yu Liu1, Mingtang Tan2, Zhongqin Chen2
1College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Zhanjiang 524088, China.
Abstract:
This study investigated the effects of extraction pH on the physicochemical, conformational, and functional properties of oyster (Crassostrea hongkongensis) protein isolates (OPIs). The results showed that with increasing extraction pH, the yield, purity, particle size, and surface hydrophobicity of OPIs significantly increased (P < 0.05), while the content of free sulfhydryl groups and the L* value significantly decreased (P < 0.05). Multispectral analysis and scanning electron microscope revealed that high pH promoted the transition from α-helix to β-sheet structures, altering the microenvironment of tryptophan residues and the microstructure of the protein. Furthermore, at extraction pH 10, OPIs exhibited optimal gel strength, water/oil absorption capacity, foaming ability, and thermal stability, indicating their suitability for gel-based and foam-based food applications. However, OPIs at extraction pH 8 exhibited the highest solubility and emulsifying capacity. These results provide a basis for the tailor-made extraction of OPIs for specific functionalities.
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