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Updated: Sep 13, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
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A Review of DNA Restriction-Free Overlapping Sequence Cloning Techniques for Synthetic Biology

Isabella Frighetto Bomfiglio1,2, Isabelli Seiler de Medeiros Mendes1,2, Diego Bonatto1,2

  • 1Laboratório de Biologia Molecular e Computacional, Centro de Biotecnologia da UFRGS, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Biotechnology Journal
|July 27, 2025
PubMed

Keywords:
DNA cloningDNA overlapping sequencesrestriction‐free cloningsynthetic biology

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