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The arthropod P450 Enchiridion: An integrated web resource for research on P450s
Jason Charamis1, Wannes Dermauw2, Thomas Van Leeuwen3
1Institute of Molecular Biology & Biotechnology, Foundation for Research & Technology Hellas, 100 N. Plastira Street, GR-70013, Heraklion, Crete, Greece.
Abstract:
We present the Arthropod P450 Enchiridion (arthropodp450.eu), a website based on the DokuWiki architecture and designed to provide resources on structures and functions of CYP genes and P450 enzymes from the most diverse and numerous animals, the arthropods. Interlinked pages cover some essential common features of P450 enzymes from rules of nomenclature to enzyme mechanism and protein structure. Specific to arthropods, lookup tables allow the rapid assignment of currently 1308 CYP families to their nine respective CYP clans, as well as the distribution of P450s from full CYPomes into clans. Manually annotated CYPomes from currently 75 species are presented with their correct CYP names as well as nearly 3000 full-length, named insect transcripts. The Arthropod P450 Enchiridion offers a BLAST function addressing over 11,000 accurately named P450 protein sequences. P450 functions as assessed by in vitro heterologous expression, RNAi phenotype, transgenic in vivo expression, or the phenotype of null mutants are presented for over 1000 cases. The data show as expected a focus on insect and mite pest species, but also highlights the remaining gaps in our knowledge of sequence and function of P450s in less studied groups of arthropods. The architecture of the DokuWiki enables regular updates and expansions to which authors are welcome to contribute.
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