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Updated: Sep 13, 2025
Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
Published on: May 14, 2012
Protocol for directed differentiation of human induced pluripotent stem cells into thyroid follicular epithelial
Hendrik J Undeutsch1, Alberto Posabella2, Darrell N Kotton3
1Division of Endocrinology, Diabetes and Metabolism, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, USA; Department of Medicine, Boston University Chobanian and Avedisian School of Medicine and Boston Medical Center, Boston, MA, USA; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, USA.
Abstract:
We provide a directed differentiation protocol deriving thyroid follicular cells (TFCs) from human induced pluripotent stem cells (iPSCs) or embryonic stem cells without exogenous transcription factors, recapitulating the sequence of developmental milestones of thyroid development. We describe a protocol for seeding iPSCs, inducing definitive and anterior foregut endoderm, followed by thyroid lineage specification via Fibroblast Growth Factor/Bone Morphogenetic Protein (FGF/BMP) signaling and subsequent differentiation and maturation into TFCs. Our protocol is validated using stem cell lines from a variety of human genetic backgrounds. For complete details on the use and execution of this protocol, please refer to Undeutsch et al.1.
