Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native
Shivraj M Yabaji1, Suruchi Lata1, Igor Gavrish1
1The National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, Boston, MA 02118, USA.
STAR Protocols
|July 27, 2025
Summary
This study details a new mouse model for investigating post-primary pulmonary tuberculosis (PTB) using Mycobacterium tuberculosis (Mtb) infection. The protocol covers infection, tissue processing, and histopathology for studying TB mechanisms.
Area of Science:
- Immunology
- Microbiology
- Pathology
Background:
- Pulmonary tuberculosis (PTB) remains a significant global health challenge.
- Understanding the mechanisms of post-primary PTB is crucial for developing effective treatments.
- Current research requires robust animal models to study virulent Mycobacterium tuberculosis (Mtb) infection.
Purpose of the Study:
- To present a detailed protocol for a novel mouse model to study post-primary pulmonary tuberculosis.
- To establish a reproducible method for Mtb infection and subsequent analysis.
Main Methods:
- Subcutaneous hock infection in mice with virulent Mtb.
- Lung and spleen implantation procedures.
- Collagen instillation, organ perfusion, tissue processing, and histopathologic interpretation.
Main Results:
- The protocol provides a comprehensive framework for establishing and analyzing a post-primary PTB mouse model.
- Detailed steps ensure reproducibility in Mtb infection and subsequent organ analysis.
Conclusions:
- This mouse model offers a valuable tool for dissecting the complex mechanisms of post-primary pulmonary tuberculosis.
- The described protocol facilitates standardized research into Mtb pathogenesis and host response.
