Related Experiment Video
Updated: Sep 13, 2025

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
Published on: January 30, 2016
Performance verification of the "ghost"-free tomographic reconstruction method QURT
Norio Baba1, Hiroyuki Ishikawa2, Miki Ito3
1School of Engineering, Kogakuin University, 2665-1 Nakano, Hachioji, Tokyo 192-0015, JAPAN.
Abstract:
We recently proposed a novel tomographic reconstruction method called the quantisation unit reconstruction technique (QURT) that reconstructs a tomogram by arranging grey-level quantisation units in three-dimensional image space through unique discrete processing. QURT significantly reduces artificial image "ghost" generation caused by the "missing-wedge" and the number of projection images, which are common limitations of conventional methods. Unlike other recently developed advanced methods, such as compressed sensing, QURT does not require any parameter adjustments or modeling based on prior knowledge but requires only a series of projection images and tilt-angle data. It does not select the processing target because it exploits the inherent constraints of projection theory. In this study, the practical performance and resolution of QURT were quantitatively evaluated in both real and frequency domains using Fourier ring correlation and other metrics in simulations with fine test patterns. Even in the presence of the missing-wedge, QURT generated almost no ghost image outside the test pattern region, considerably suppressing the artifacts. This advanced property was experimentally validated by applying HAADF-STEM tomography to analyse a catalyst sample (Pd/CeO2-ZrO2-Al2O3), which was particularly sensitive to ghost image generation inside pores. QURT clearly revealed the pore structure without compromising the low-contrast Al regions and without generating ghost images, even at a tilt step angle of up to 4°. In EDXS tomography, QURT also reconstructed a 3D elemental map even from a small number of 22 tilt series signal maps with the missing-wedges.
Related Concept Videos
Computed Tomography
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Studies III: Computed Tomography
Positron Emission Tomography
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

