Related Experiment Video
Updated: Sep 13, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
OsBZR4 regulates temperature-dependent embryogenesis in rice
Zhenyu Wang1, Min Xu1,2, Yingxiang Liu1,2
1Northeast Institute of Geography and Agroecology, State Key Laboratory of Black Soils Conservation and Utilization, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, China.
Abstract:
Embryoless rice is valuable for studying early seed development and has great breeding potential, however, related research remains limited. Here, we show that mutations in OsBZR4, encoding brassinazole-resistance 4, lead to 60-100% embryoless seeds across different cultivars. OsBZR4 is specifically expressed at the scutellum-endosperm interface and regulates auxin levels and distribution during early embryo development. OsBZR4 represses the expression of YUC4 and PIN5b. Exogenous auxin and overexpression of YUC4 enhance the embryoless ratio, whereas the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) decrease it. Higher temperatures increase the embryoless ratio in bzr4 mutant lines. The expression of OsPIL13 is induced by high temperature, and its overexpression enhances the embryoless ratio in both wild type and bzr4 plants. Introducing bzr4 mutant allele into some elite cultivars can improve milled rice yield and storability. Collectively, manipulating OsBZR4 can lead to the development of thermosensitive embryoless rice varieties with increased energy reserves and improved storability, potentially enabling novel rice production technologies.
More Related Videos
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
07:32Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
Published on: June 9, 2015
Related Concept Videos
Osmoregulation in Insects
Gene Regulation During Sporulation
Regulation of Transpiration by Stomata
Osmoregulation in Fishes
Responses to Heat and Cold Stress
Responses to Drought and Flooding