Au-doped MoS₂ nanozyme with self-cascade catalytic performance for one-step glucose detection application

Area of Science:

• Nanomaterials Science
• Biochemistry
• Analytical Chemistry

Background:

• Glucose oxidase (GOx) is crucial for biosensors in diabetes and tumor screening.
• Existing GOx methods are costly and involve complex procedures.
• Need for efficient, cost-effective glucose detection strategies.

Purpose of the Study:

• To develop a facile, green synthesis of Au-doped MoS2 nanomaterials.
• To engineer a nanozyme with dual-enzyme activities (GOx-like and peroxidase-like).
• To enable a one-step colorimetric glucose detection method.

Main Methods:

• Green aqueous-phase synthesis of Au-doped MoS2 nanomaterials.
• Characterization of the hybrid nanostructure and its catalytic activities.
• Development of a one-step colorimetric assay for glucose detection.

Main Results:

• Au-MoS2 nanozyme exhibited dual-enzyme activities.
• Achieved a one-step colorimetric glucose detection with a wide linear range.
• Demonstrated an ultralow detection limit of 0.09 mM.
• Validated in biological samples (cancer cells, diabetic urine).

Conclusions:

• The Au-MoS2 nanozyme offers a simplified, cost-effective glucose detection method.
• Shows significant potential for clinical diagnostics and disease screening.
• Provides a stable and reproducible platform for biosensing applications.