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Updated: Sep 8, 2025

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Investigations on semen analysis capabilities and quality control measures in laboratories with different management
Hong-Lin Li1, Jin-Chun Lu2, Chao Zhao1
1Center for Reproductive Medicine, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu, 223300, China.
Purpose:
To perform a survey on the semen analysis capabilities and quality control measures of medical institutions conducting male infertility-related testing in Jiangsu, China, by the WeChat, aiming to explore the possibility of mutual recognition of testing results among different laboratories.
Methods:
This study designed a survey questionnaire consisting of 105 questions and investigated and compared semen analysis capabilities and quality control measures between regulated laboratories for assisted reproductive technology (RLART) and non-regulated laboratories for assisted reproductive technology (non-RLART) in Jiangsu, China.
Results:
RLART had higher percentages of technicians received training on the WHO manual (97.14% [34/35] vs. 57.14% [28/49], P < 0.01), dedicated rooms for semen collection (100% [35/35] vs. 24.49% [12/49], P < 0.01), adherence to the staining methods recommended by the WHO manual (100% [32/32] vs. 33.33% [11/33], P < 0.01), internal quality control for sperm concentration (77.14% [27/35] vs. 6.12% [3/49], P < 0.01), and participation in external quality assessment (74.29% [26/35] vs. 14.29% [7/49], P < 0.01) compared to non-PLART.
Conclusions:
Establishing unified recognition criteria for semen analysis laboratories and conducting regular supervision can improve semen analysis capabilities and promote mutual recognition of semen testing results.
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