Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.3K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medializing the Reaming Entry Point for Pertrochanteric Femur Fractures Treated with Cephalomedullary Nails: A Technical Trick.

Journal of orthopaedic trauma·2026
Same author

Z-type Clavicle Fractures in Adults: A Retrospective Comparative Study of Outcomes.

The Journal of the American Academy of Orthopaedic Surgeons·2026
Same author

TREM2 restrains myeloid inflammasome activation to protect against photoreceptor degeneration.

Journal of neuroinflammation·2026
Same author

Risk Factors for Failure of Repair of Diaphyseal Tibial Nonunions: A Retrospective Cohort Study of 108 Patients.

Injury·2026
Same author

Efficacy of anti-VEGF single-chain variable fragment AAV-based gene therapy in a laser-induced choroidal neovascularisation mouse model.

Molecular therapy. Advances·2026
Same author

Implementation of a wellness program in a subspecialized community radiology practice arm of a large academic healthcare system.

Clinical imaging·2026

Related Experiment Video

Updated: Sep 13, 2025

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
07:32

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

14.5K

TREM2-Mediated Myeloid Cells Protect Against Pathological Choroidal Neovascularization.

Tianxi Wang1, Manon Szczepan1, Austin T Gregg1

  • 1Department of Ophthalmology, Harvard Medical School, Boston Children's Hospital, Boston, Massachusetts, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|July 27, 2025
PubMed
Summary

Triggering receptor expressed on myeloid cells 2 (TREM2) plays a protective role in choroidal neovascularization (CNV). Its deficiency worsens CNV, highlighting TREM2

Keywords:
SOCS3Trem2choroidal neovascularizationmacrophagesmicrogliamyeloid cells

More Related Videos

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research
04:43

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

Published on: June 16, 2023

1.1K
Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report
10:24

Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report

Published on: February 12, 2018

10.3K

Related Experiment Videos

Last Updated: Sep 13, 2025

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
07:32

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

14.5K
Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research
04:43

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

Published on: June 16, 2023

1.1K
Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report
10:24

Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report

Published on: February 12, 2018

10.3K

Area of Science:

  • Ophthalmology
  • Immunology
  • Genetics

Background:

  • Choroidal neovascularization (CNV) causes irreversible vision loss in age-related macular degeneration.
  • Myeloid cell activation is implicated in CNV, but molecular mechanisms are unclear.
  • Triggering receptor expressed on myeloid cells 2 (TREM2) is an immunomodulatory receptor with known protective roles in other diseases.

Purpose of the Study:

  • To investigate the role of TREM2 in the pathogenesis of CNV.
  • To elucidate the molecular mechanisms by which TREM2 influences myeloid cell function in CNV.

Main Methods:

  • Single-cell RNA sequencing to identify TREM2 expression in CNV.
  • Immunostaining to validate TREM2 protein levels.
  • Functional studies using Trem2 haploinsufficient and compound mutant mice.
  • Transcriptomic profiling of TREM2-expressing myeloid cells.

Main Results:

  • TREM2 is selectively upregulated in activated microglia and macrophages in laser-induced CNV.
  • TREM2 deficiency exacerbates CNV lesion size and vascular leakage.
  • TREM2-expressing myeloid cells show distinct angiogenic and inflammasome gene signatures.
  • TREM2 and SOCS3 function independently but cooperatively in anti-angiogenic pathways.

Conclusions:

  • TREM2 plays a protective role in CNV pathogenesis.
  • TREM2 regulates angiogenesis by modulating inflammatory pathways in myeloid cells.
  • TREM2 is a key regulator of myeloid cell function in the diseased retina.