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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
Published on: June 20, 2025
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Neuroinflammation and immune microenvironment alterations following heat stroke: A transcriptomic perspective
Kuan-Nien Chou1, Xin-Jie Lu2, Hsing-Fan Lai3
1Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 114, Taiwan, ROC; Department of Neurological Surgery, Tri-service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Computers in Biology and Medicine
|July 27, 2025
Summary
Heat stroke (HS) causes sterile neuroinflammation by increasing immune cells and activating microglia in the brain. This acute injury may share pathways with chronic neurodegenerative diseases.
Area of Science:
- Neuroscience
- Immunology
- Toxicology
Background:
- Heat stroke (HS) is a critical condition causing severe sterile neuroinflammation in the brain.
- Understanding the brain's immune response during HS is crucial for developing effective treatments.
Purpose of the Study:
- To characterize the immune microenvironment in the brain during heat stroke using transcriptomic profiling.
- To investigate potential links between heat stroke-induced neuroinflammation and chronic neurodegenerative conditions.
Main Methods:
- Transcriptomic profiling of murine brain tissue.
- Gene set enrichment and cell deconvolution analyses.
- Immunohistochemical studies to validate transcriptomic findings.
Main Results:
- Heat stroke significantly altered gene expression, upregulating innate immune and inflammatory pathways.
- Increased infiltration of peripheral myeloid cells (granulocytes, monocytes/macrophages) and activated microglia were observed.
- Downregulation of neuronal genes and complex immune responses with both pro- and anti-inflammatory markers were noted.
Conclusions:
- Heat stroke induces acute sterile neuroinflammation and neurodegeneration, involving a complex interplay of immune cells.
- The study suggests potential parallels between HS neuroinflammation and pathways implicated in Alzheimer's disease.
- HS serves as a valuable model for studying acute sterile neuroinflammation and its implications for neurodegenerative diseases.
