Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osmoregulation in Insects01:47

Osmoregulation in Insects

Malpighian tubules are specialized structures found in the digestive systems of many arthropods, including most insects, that handle excretion and osmoregulation. The tubules are typically arranged in pairs and have a convoluted structure that increases their surface area.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence Map Analysis on Randomized Controlled Trials: Chinese Patent Medicines and Classic Traditional Chinese Medicine Prescriptions.

Journal of evidence-based medicine·2026
Same author

DCAF13 is associated with pancreatic ductal adenocarcinoma progression and p53-related signaling in TP53-mutant cells.

Biochemical and biophysical research communications·2026
Same author

YIF1A activates mTORC1 signaling to promote cellular senescence.

Cell death & disease·2026
Same author

A study on risk prediction of decline in self-care ability one month after discharge in postoperative colorectal cancer patients based on routine clinical indicators.

Frontiers in surgery·2026
Same author

SERS Facemask for Rapid and Portable Sensing Mycobacterium Tuberculosis Antigens for TB Screening.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Efficacy and Implementation of a Theory-Underpinning Needs-Oriented mHealth Supportive Program for Family Caregivers of Community-Dwelling Frail Older Adults (Hi-Care): A Randomized Controlled Trial.

The American journal of geriatric psychiatry : official journal of the American Association for Geriatric Psychiatry·2026
Same journal

Males are worse mothers: Comparing care patterns in a facultatively caring beetle.

Insect science·2026
Same journal

RNAi-based functional genomics tools for the beet leafhopper using microinjection and nanoparticle-based topical spray.

Insect science·2026
Same journal

Global expansion, insecticide resistance, and the future of integrated pest management for Megalurothrips usitatus: A comprehensive review.

Insect science·2026
Same journal

Drosophila Nup358 functions as a SUMO E3 ligase to promote the SUMOylation and nuclear import of the juvenile hormone receptor Methoprene-tolerant.

Insect science·2026
Same journal

Mre11 deficiency leads to fat body senescence through concurrent dysregulation of mitochondrial function and lipid metabolism.

Insect science·2026
Same journal

A myrmecophilous beetle associated with Solenopsis invicta in China: Evidence for potential host-associated co-dispersal.

Insect science·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits
12:13

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

Published on: January 25, 2013

27.2K

R-loop modulates odor-guided behavior in migratory locusts.

Jipeng Liu1,2, Lihan Zhao1, Jiaqi Yu1

  • 1College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.

Insect Science
|July 28, 2025
PubMed
Summary
This summary is machine-generated.

Excessive R-loops, structures that can impair genome stability, disrupt olfactory responses in locusts. The enzyme RNase H1 (RNH1) resolves these R-loops, and its deficiency leads to loss of smell.

Keywords:
RNase H1R‐loopmigratory locustolfaction

More Related Videos

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
09:30

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Published on: July 13, 2017

8.2K
Single Sensillum Recordings for Locust Palp Sensilla Basiconica
07:16

Single Sensillum Recordings for Locust Palp Sensilla Basiconica

Published on: June 23, 2018

8.4K

Related Experiment Videos

Last Updated: Jun 23, 2026

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits
12:13

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

Published on: January 25, 2013

27.2K
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
09:30

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Published on: July 13, 2017

8.2K
Single Sensillum Recordings for Locust Palp Sensilla Basiconica
07:16

Single Sensillum Recordings for Locust Palp Sensilla Basiconica

Published on: June 23, 2018

8.4K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • R-loops (DNA:RNA hybrids) are implicated in neurodegenerative diseases and genome instability.
  • Olfactory dysfunction is an early symptom of neurodegenerative disorders, but the role of R-loops in olfaction is unclear.
  • Migratory locusts exhibit olfactory responses to conspecifics and plants, making them a suitable model.

Purpose of the Study:

  • To investigate the function of R-loops in modulating olfaction in the central nervous system of migratory locusts.
  • To determine the role of RNase H1 (RNH1) in resolving R-loops and its impact on olfactory behavior.

Main Methods:

  • Immunofluorescence analysis to detect R-loops and RNH1 expression in locust antennae and brains.
  • RNA interference (RNAi) to knock down RNH1 expression.
  • Behavioral assays to assess olfactory responses to odorants.
  • Assessment of DNA damage and nascent RNA synthesis.

Main Results:

  • R-loops and RNH1 are present in the locust antenna and brain, particularly in mushroom bodies and antennal lobes.
  • RNH1 knockdown resulted in loss of olfactory responses without affecting locomotion or body color.
  • RNH1 deficiency led to increased R-loop levels, DNA damage, and decreased nascent RNA synthesis in the brain.

Conclusions:

  • Increased R-loop levels due to RNH1 deficiency may cause the loss of olfactory responses in locusts.
  • This study is the first to demonstrate the involvement of RNH1-mediated R-loop resolution in olfactory behavioral regulation.
  • Findings provide new insights into the role of R-loops in modulating olfaction and potential implications for neurodegenerative diseases.