Related Experiment Video
Updated: Sep 13, 2025

08:15
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
Published on: October 6, 2018
50.8K
Studies of Release Activity in DRGs of Rats with Spared Nerve Injury (SNI) Using YO-PRO-1
Yanping Gu1, Guangwen Li1, Li-Yen Mae Huang1
1Neurobiology, University of Texas Medical Branch.
Micropublication Biology
|July 28, 2025
Abstract:
We report a new method for examining changes in membrane permeability in dorsal root ganglion (DRG) cells under peripheral nerve injury conditions. By intravenously injecting YO-PRO-1, a monomeric cyanine nucleic acid stain, into rats with spared nerve injury (SNI), we observed the uptake of YO-PRO-1 in injured neurons and surrounding non-neuronal cells. This approach provides a tool for detecting in vivo DRG cell release activity in intact animals.

