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Updated: Jun 27, 2026

Aseptic Laboratory Techniques: Plating Methods
Published on: May 11, 2012
Phytopathogenic bacterial survival on artificial substrates
Emily E Pfeufer1,2, Aaron J Sechler1, Matthew A Tancos1
1Foreign Disease-Weed Science Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Frederick, MD.
Abstract:
The ability of phytopathogenic bacteria to survive desiccation on inanimate substrates has important implications for managing potential contamination and resulting bacterial spread during both real-world horticultural operations and laboratory experimentation. Here we demonstrate that Pseudomonas marginalis, Xanthomonas campestris, Rathayibacter agropyri, and R. iranicus are all capable of surviving desiccation on both polystyrene plastic and glass surfaces and that the likelihood of survival increases with increasing initial bacterial concentration. X. campestris was recovered at higher frequencies from plastic than from glass, while the other species were recovered at roughly equal frequencies from each surface.
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