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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
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Toward Bioactive Hydrogels: A Tunable Approach via Nucleic Acid-Collagen Complexation.

Nikolaos Pipis1, Senthilkumar Duraivel2, Vignesh Subramaniam3

  • 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.

Regenerative Engineering and Translational Medicine
|July 28, 2025
PubMed
Summary
This summary is machine-generated.

Nucleic acid-collagen complexes (NACCs) form tunable hydrogels. DNA addition reinforces collagen networks, enabling control over elasticity for biomedical applications.

Keywords:
AptamersCollagenDNA-collagen complexesMicrofibersRheologyTunable biomaterials

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Area of Science:

  • Biomaterials Science
  • Biochemistry
  • Materials Engineering

Background:

  • Nucleic acid-collagen complexes (NACCs) are biomaterials formed by binding single-stranded DNA (ssDNA) with type I collagen.
  • These complexes self-assemble into microfibers and nanoparticles, showing potential in tissue engineering and regenerative medicine.
  • The precise mechanisms of nucleic acid-driven collagen assembly and their impact on material properties require further elucidation.

Purpose of the Study:

  • To investigate the relationship between the microscopic structure of NACCs and their bulk material properties.
  • To demonstrate that NACCs can be engineered as mechanically tunable systems by controlling collagen-DNA interactions.
  • To understand how varying ratios of collagen to ssDNA influence NACC formation and properties.

Main Methods:

  • Characterization of NACCs using varying molar ratios of collagen to random ssDNA oligonucleotides.
  • Assessment of molecular interactions via infrared spectroscopy.
  • Evaluation of gelation and rheological behavior.
  • Microscopic analysis using phase contrast, confocal reflectance, and transmission electron microscopy to determine 3D structural organization.

Main Results:

  • DNA oligonucleotides significantly reinforce and rearrange the collagen hydrogel network.
  • DNA addition accelerates gelation by promoting rapid fiber formation and spontaneous self-assembly.
  • The elasticity of NACC hydrogels is tunable based on the collagen-to-DNA molar ratio, ssDNA length, and collagen type.

Conclusions:

  • The study demonstrates the successful engineering of mechanically tunable DNA-based hydrogel systems.
  • Tailoring DNA content and collagen concentration allows for precise control over hydrogel stiffness.
  • These findings offer new possibilities for developing advanced bioactive hydrogels for diverse biomedical applications.