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Updated: Jul 28, 2026
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
Knockdown of CSF1R molecules enhances the antitumor effects of CD8+ T lymphocytes in bladder cancer
Jingxuan Peng1,2, Dongjie Li1,2, Boyu Xiang1,2
1Department of Urology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, China.
Objective:
In this study, we preliminarily investigated the efffects of the colony stimulating factor 1 receptor (CSF1R) molecules on CD8+ T cells in bladder cancer (BLCA) and the underlying molecular mechanism.
Methods:
The effects of CSF1R in CD8+ T cells on BLCA cell proliferation, migration and, invasion were determined by cell-killing assay and transwell assays.For in vivo experiments, tumours tissues were divided into four portions: (1) histological staining, (2) flow cytometry detection, (3) mRNA gene sequencing analysis, and (4) qPCR validation. Information related to 161 bladder cancer patients with BLCA at Xiangya Hospital of Central South University from 2019 to 2023 was collected. The pathological tissues were subjected to immunofluorescence. By calculating the Jordon index, CD8+ T cells with CSF1R expression at or above 0.39-fold were included in the high-expression group.
Results:
Knocking down of CSF1R in CD8+ T cells inhibited BLCA proliferation, migration, and invasion. Flow cytometry revealed that it could lead to increased infiltration of immune cells. mRNA sequencing, quantitative polymerase chain reaction (PCR) and Western blot (WB) results suggested that creatine kinase (Ckm) was significantly decreased after CSF1R knockdown.
Conclusion:
Our study provides novel insights into the roles of CSF1R in BLCA progression and the underlying crosstalk between tumour metabolism and the immune microenvironment.
Insights
Colony stimulating factor 1 receptor (CSF1R) in CD8+ T cells suppresses bladder cancer progression. Targeting CSF1R enhances anti-tumor immunity and reduces cancer cell invasion, offering a potential therapeutic strategy.
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- Bladder cancer (BLCA) remains a significant health concern.
- The role of colony stimulating factor 1 receptor (CSF1R) in CD8+ T cells within the tumor microenvironment is not fully understood.
- Investigating immune cell function is crucial for developing novel cancer therapies.
Purpose of the Study:
- To investigate the effects of CSF1R on CD8+ T cells in bladder cancer.
- To elucidate the molecular mechanisms underlying CSF1R's influence on tumor progression.
- To explore the potential of targeting CSF1R for bladder cancer treatment.
Main Methods:
- Cell-based assays (killing, transwell) to assess BLCA proliferation, migration, and invasion.
- In vivo tumor tissue analysis including histological staining, flow cytometry, mRNA sequencing, and qPCR.
- Patient data from 161 BLCA cases collected between 2019-2023.
- Immunofluorescence and Jordan index calculation to define high CSF1R expression groups.
Main Results:
- Knockdown of CSF1R in CD8+ T cells significantly inhibited BLCA cell proliferation, migration, and invasion.
- CSF1R knockdown led to increased immune cell infiltration in tumor tissues.
- mRNA sequencing and qPCR revealed a significant decrease in creatine kinase (Ckm) expression post-CSF1R knockdown.
Conclusions:
- CSF1R plays a critical role in bladder cancer progression.
- There is a significant crosstalk between tumor metabolism and the immune microenvironment mediated by CSF1R.
- Targeting CSF1R in CD8+ T cells presents a promising avenue for enhancing anti-tumor immunity in BLCA.
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