A Surgical Approach for Optic Nerve Crush in a Rabbit Model

Area of Science:

• Ophthalmology
• Neuroscience
• Surgical Innovation

Background:

• Optic nerve crush (ONC) models are crucial for studying optic neuropathies.
• Rodent models have limitations in human translatability due to anatomical and physiological differences.
• A more human-relevant animal model is needed for optic nerve injury research.

Purpose of the Study:

• To describe a novel surgical technique for creating an ONC model in rabbits.
• To validate the rabbit ONC model using functional assessments.
• To establish a translatable model for optic nerve injury research.

Main Methods:

• A minimally invasive surgical technique for ONC in rabbits was developed, adapting existing ophthalmic surgical procedures.
• The model was validated by comparing pupillary light responses and visual evoked potentials (VEPs) between injured and contralateral eyes.
• No bone windows or canthotomies were required for the surgical approach.

Main Results:

• The rabbit ONC model demonstrated a significant reduction in pupillary light response post-injury.
• VEP amplitudes were significantly decreased in ONC eyes compared to controls, confirming visual function loss.
• The results indicate successful induction of optic nerve damage and functional deficit.

Conclusions:

• The described surgical technique provides a viable and translatable rabbit model for optic nerve crush.
• This model can be used to investigate optic neuropathies and evaluate therapeutic interventions.
• The rabbit ONC model has implications for advancing treatments for ocular neurodegenerative diseases.