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Receptor-mediated Endocytosis01:38

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Related Experiment Video

Updated: Jul 17, 2026

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation
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Resveratrol-Loaded PLGA Enhanced Vitrified Oocyte Viability through Rab11fip4/Arf5-Mediated Internalization Route.

Yixiao Zhu1, Jun Li2, Guizhen Zhou1

  • 1State Key Laboratory of Animal Biotech Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the MARA, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

ACS Applied Materials & Interfaces
|July 28, 2025
PubMed
Summary

Resveratrol-loaded nanoparticles protect vitrified oocytes by enhancing endocytosis via the Rab11fip4 pathway. This nanotechnology approach improves oocyte viability and maturation, addressing challenges in cryopreservation.

Keywords:
Rab11fip4endocytosisnanoparticlesoocytesvitrification

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Area of Science:

  • Reproductive Biology
  • Nanotechnology
  • Cellular Mechanisms

Background:

  • Oocyte vitrification challenges remain significant, impacting fertility treatments.
  • Nanomaterials show promise for protecting cells during cryopreservation.
  • Molecular mechanisms of nanoparticle protection in vitrified oocytes are not well understood.

Purpose of the Study:

  • To synthesize resveratrol-loaded poly(lactic-co-glycolic acid) nanoparticles (PLGA-RES).
  • To investigate the protective effects and underlying mechanisms of PLGA-RES on vitrified mouse oocytes.
  • To elucidate the role of Rab11fip4 in nanoparticle internalization and oocyte quality.

Main Methods:

  • Synthesis and characterization of PLGA-RES nanoparticles using FTIR.
  • Assessment of nanoparticle internalization in mouse oocytes via TEM.
  • Evaluation of oocyte quality (GVBD rate, aneuploidy rate).
  • Transcriptome analysis (STEM, WGCNA), molecular docking, co-immunoprecipitation (co-IP), and endocytic inhibitor assays.

Main Results:

  • PLGA-RES nanoparticles were successfully synthesized and internalized by oocytes in a time- and dose-dependent manner.
  • PLGA-RES treatment significantly improved oocyte maturation (increased GVBD) and reduced aneuploidy.
  • Transcriptome analysis revealed the endocytosis pathway's importance, identifying Rab11fip4 as a key regulator of nanoparticle internalization and function.
  • Rab11fip4 mediates PLGA-RES uptake and protects them from lysosomal degradation via the recycling endosome pathway.

Conclusions:

  • PLGA-RES nanoparticles enhance vitrified oocyte viability and quality through the endocytic pathway.
  • Rab11fip4 plays a critical role in nanoparticle internalization and functional modulation in oocytes.
  • This study provides insights into nanoparticle-mediated protection mechanisms for oocyte cryopreservation.