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Updated: May 4, 2026
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Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
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Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications
Christine L Trautmann1, Adhideb Ghosh1,2, Ali Kerem Kalkan1
1Laboratory of Regenerative and Muscle Biology, Institute of Human Movement Sciences and Sport, Department of Health Sciences and Technology, ETH Zurich, Schwerzenbach, 8603, Switzerland.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 28, 2025
Summary
Researchers enhanced bovine myogenic progenitor cell (MPC) differentiation for cultivated meat using novel media. This molecularly characterized method improves in vitro muscle cell development for sustainable meat alternatives.
Area of Science:
- Biotechnology
- Cell Biology
- Sustainable Agriculture
Background:
- Conventional livestock farming presents environmental, health, and animal welfare issues.
- Cultivated meat offers a sustainable alternative, relying on myogenic progenitor cell (MPC) differentiation into muscle cells.
- Limited understanding exists regarding the molecular mechanisms controlling MPC differentiation and potential enhancement strategies.
Purpose of the Study:
- To characterize the molecular landscape of bovine MPC differentiation in vitro.
- To explore augmentation of MPC differentiation using small molecules and novel media formulations.
- To develop improved methods for cultivated meat production.
Main Methods:
- Employed multiomics (bulk and single-cell transcriptomics, proteomics) to analyze bovine MPC differentiation.
- Compared conventional and novel enhanced differentiation media in 2D cultures and 3D skeletal muscle constructs.
- Identified unique cell populations and molecular markers associated with enhanced differentiation.
Main Results:
- Novel media significantly enhanced myogenic differentiation compared to conventional methods.
- Enhanced media promoted the development of progenitor-like cells, myocytes, and contractile myotubes.
- The resulting cells more closely resembled in vivo bovine muscle cells, expressing key myogenic markers.
- A detailed molecular roadmap of enhanced differentiation was established.
Conclusions:
- The developed enhanced media and multiomics approach significantly improve in vitro myogenic differentiation.
- This method provides a valuable molecular roadmap for advancing cultivated meat technology.
- The findings support the potential for more sustainable and efficient cultivated meat production.
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