Durable formulations of quorum quenching enzymes

Enzymes with industrial potential often face limitations due to stability and longevity constraints. Thermostable quorum quenching lactonases are appealing biotechnology tools for controlling microbial pathogenicity and biofilm formation via the interference of quorum sensing. However, the effective formulation of these enzymes remains a challenge. Here, we evaluate the resistance and activity of two thermostable quorum quenching lactonase enzymes (SsoPox and GcL) across diverse formulations relevant to industrial applications. We systematically tested these enzymes with 16 different crop adjuvants (including oils, an anti-foaming agent, surfactants, deposition aids, a water conditioner, and a sticking agent) over a 210-day period, demonstrating broad compatibility except with oil-based adjuvants. Additionally, both enzymes maintained their activity when incorporated into five different coating bases (acrylic, silicone, polyurethane, epoxy, and latex) with activity levels varying according to polymer type. Further investigation of enzymatic acrylic coating characterized the effects of salt water and temperature on enzyme activity levels. Functionalized coatings maintained remarkable stability over 250 days in both wet and dry conditions. These findings establish a practical demonstration and framework for integrating quorum quenching lactonases into industrial materials and formulations, significantly advancing their potential for 'real-world' applications for microbial control across multiple sectors.