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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

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Updated: Sep 13, 2025

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

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Published on: November 17, 2009

Mapping rat lncRNA Bdnf-as.

Elizaveta V Shaburova^1,2, Sergey A Marchenko^1,3, Veronika B Dneprovskaya^1,3

¹Laboratory of Neurobiology and Behavior, Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk, Russian Federation.

Molecular Biology Reports

|July 28, 2025

Summary

This summary is machine-generated.

This study identifies two novel long noncoding RNA (lncRNA) transcripts, Bdnf-as, in rats that regulate Bdnf gene expression. These rat lncRNAs show limited conservation across species and are potentially unstable.

Keywords:

Bdnf-as Brain lncRNA

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Area of Science:

Neurobiology
Genomics
Molecular Biology

Background:

Long noncoding RNAs (lncRNAs) are crucial gene expression regulators, but their roles in non-human species are understudied.
This research focuses on the rat lncRNA, Bdnf-as, which is known to modulate the Bdnf gene via chromatin remodelers.

Purpose of the Study:

To identify and characterize novel antisense transcripts of Bdnf-as in the rat genome.
To investigate the expression patterns and potential regulatory functions of rat Bdnf-as.

Main Methods:

Utilized step-out rapid amplification of cDNA ends, lentivirus infusion, TET-on system, and de novo transcriptome assembly.
Employed quantitative polymerase chain reaction (qPCR) and bioinformatics analyses for transcript identification and protein binding prediction.
Conducted comparative genomic analysis to assess evolutionary conservation.

Main Results:

Identified two novel, spliced antisense transcripts of Bdnf-as downstream of the Bdnf coding region.
Observed augmented Bdnf-as expression upon proBDNF induction, suggesting regulation by BDNF pathway.
Predicted binding of several proteins (E2F1, VDR, SP3, ZNF354C, YY2, SPI1, RUNX1, TBX3) and found limited evolutionary conservation of Bdnf-as.

Conclusions:

Provides the first characterization of rat Bdnf-as, including its differential expression.
Establishes a foundation for future research into the regulatory roles and protein interactions of Bdnf-as in neurobiology.