Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

602
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
602
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

1.1K
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
1.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond Color: Hybrid Vibrational-Electronic Broadband Coherent Anti-Stokes Raman Scattering for Molecularly Informed Digital Pathology.

Analytical chemistry·2026
Same author

A novel method to simultaneously estimate bacterial respiration and growth from oxygen dynamics.

ISME communications·2026
Same author

Papaver S-determinants trigger mitochondrially derived ROS production and disrupt energy metabolism in incompatible pollen tubes.

The Plant cell·2026
Same author

Solvent Exclusion Effect on Infrared Absorption Spectroscopy.

Analytical chemistry·2025
Same author

A Liquid-Core Fiber Platform for Classical and Entangled Two-Photon Absorption Measurements.

ACS photonics·2025
Same author

Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.

Journal of experimental botany·2025

Related Experiment Video

Updated: Sep 13, 2025

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.1K

Chemical Fingerprint Imaging In Planta with Broadband Coherent Anti-Stokes Raman Scattering Microscopy.

Paul Ebersbach1, Nicholas Smirnoff2, Charles H Camp3

  • 1School of Physics and Astronomy, University of Exeter, Exeter EX4 4QL, U.K.

Analytical Chemistry
|July 29, 2025
PubMed
Summary

Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy enables high-resolution, large-area plant imaging by rapidly acquiring vibrational spectra. This advanced technique reveals detailed chemical and structural information across plant tissues, crucial for plant and agrochemical research.

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

13.8K

Related Experiment Videos

Last Updated: Sep 13, 2025

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.1K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

13.8K

Area of Science:

  • Plant science
  • Chemical imaging
  • Microscopy

Background:

  • Spontaneous Raman microscopy links molecular and cellular levels in plants.
  • Low Raman scattering probability limits resolution and speed in biological applications.
  • Absorption and autofluorescence interfere with traditional Raman methods in plant samples.

Purpose of the Study:

  • To apply broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to plant samples for the first time.
  • To overcome limitations of spontaneous Raman microscopy in speed and sensitivity for plant imaging.
  • To enable high-resolution, large-area chemical imaging of diverse plant tissues.

Main Methods:

  • Application of broadband coherent anti-Stokes Raman scattering (BCARS) microscopy.
  • Development of an optimized unmixing procedure combined with robust preprocessing.
  • Analysis of vibrational signals from leaf cross sections, from cuticle to mesophyll.

Main Results:

  • BCARS microscopy achieved fast acquisition times (10 ms/spectrum) for large-area, high-resolution imaging.
  • Selective extraction of chemical components including waxes, pectin, cellulose, chlorophyll, carotenoids, and starch.
  • Visualization of calcium oxalate crystals, flavonols, and anthocyanins in vacuoles.
  • Detection of photosystem-specific spectral changes indicating chloroplast adaptation to light gradients.
  • Identification of mixed hydration states in calcium oxalate crystals via OH-stretching signals.

Conclusions:

  • BCARS microscopy provides a powerful tool for detailed chemical and structural analysis of plant systems.
  • The method facilitates system-view imaging, advancing plant biology and agrochemical research.
  • BCARS microscopy overcomes previous limitations, enabling comprehensive analysis of complex plant tissues.