Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Medium Optimization01:28

Methods of Medium Optimization

70
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
70

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AAV vector production in suspension cells using PEI transfection and sodium butyrate with orthogonal assessment of function and quality.

Molecular therapy. Advances·2026
Same author

Dual Role of Cancer Epithelial-Specific TRAF3 in Regulating Breast Cancer Cell Survival and Lymphocyte Activity.

International journal of molecular sciences·2026
Same author

AID and TET2 cooperate to demethylate Irf4 for plasma cell fate in germinal center B cells.

The Journal of experimental medicine·2026
Same author

Serial intravital microscopy reveals temporal dynamics of autoreactive germinal centers in the spleen.

iScience·2026
Same author

Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome.

Journal of human immunity·2026
Same author

Curbing Autoimmunity: A New Fab Fragment Targeting CD40-CD40L Halts B-Cell Activation and Differentiation.

European journal of immunology·2026

Related Experiment Video

Updated: May 5, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.2K

Multiparametric Optimization of Human Primary B-Cell Cultures Using Design of Experiments.

Anne Bruun Rovsing1,2,3, Kenneth Green1,4,5, Lisbeth Jensen1

  • 1Department of Biomedicine, Aarhus University, Aarhus, Denmark.

Scandinavian Journal of Immunology
|July 29, 2025
PubMed
Summary

This study optimized a human B-cell culture system, identifying CD40L and IL-4 as critical for B-cell function and antibody production. The findings advance understanding of human immune responses.

Keywords:
B‐cell cultureCD40LIL‐4

More Related Videos

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

8.6K
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

6.4K

Related Experiment Videos

Last Updated: May 5, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.2K
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
09:28

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

8.6K
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

6.4K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • B cells are crucial for adaptive immunity, mediating antibody production and immune memory.
  • Mouse models inform B-cell mechanisms, but human relevance requires specific investigation.

Purpose of the Study:

  • To develop and optimize a human primary B-cell culture system for studying B-cell activation and antibody responses.
  • To dissect the roles of CD40L, BAFF, IL-4, and IL-21 in human B-cell function.

Main Methods:

  • Utilized a human primary B-cell culture system with engineered feeder cells expressing CD40L.
  • Supplemented cultures with BAFF, IL-4, and IL-21, employing a Design of Experiments (DOE) approach for parameter optimization.

Main Results:

  • CD40L and IL-4 were identified as critical for human B-cell viability, proliferation, and IgE class-switching.
  • BAFF showed a negligible role, while IL-21 had subtle effects.
  • Engineered feeder cells and purified cytokines offered comparable and flexible system modulation.

Conclusions:

  • The optimized human B-cell culture system provides a robust platform for investigating B-cell biology and antibody regulation.
  • This system supports in vitro production of human primary antibodies, advancing therapeutic and research applications.