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Updated: Jun 19, 2026
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
Published on: April 4, 2016
Do Preparation Techniques Transform the Metabolite Profile of Platelet-Rich Plasma?
Bilge Başak Fidan1, Emine Koç2, Emine Çiftçi Özotuk3
1Department of Bioengineering, Graduate School of Science and Engineering, Hacettepe University, Ankara 06800, Türkiye.
Background:
Platelet-rich plasma (PRP) is a widely used therapeutic product in musculoskeletal treatments due to its regenerative and anti-inflammatory properties. However, the lack of standardization in PRP preparation protocols hampers clinical consistency.
Methods:
In this study, the metabolic profiles of 10 different PRP types were compared using untargeted metabolomics via Q-TOF LC-MS. PRP-G and PRP-S were prepared from six donors to assess inter-individual variability, while the remaining types were obtained from a single donor to isolate the impact of preparation method alone. Multivariate analyses, VIP scores, and pathway enrichment analyses were conducted.
Results:
PRP formulations exhibited distinct metabolic differences associated with inflammatory signaling, redox homeostasis, steroid metabolism, energy production, and platelet activation. Samples from both single- and multi-donor groups showed high intra-group similarity, indicating that preparation method is a major determinant of PRP's biochemical composition.
Conclusion:
Metabolomic profiling reveals that even minor differences in PRP preparation protocols can lead to significant biochemical changes that may affect therapeutic outcomes. This study highlights the need for standardized, indication-specific PRP products and underscores the value of metabolomic analysis in guiding optimal formulation selection in clinical practice.
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