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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

14.8K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.8K
Transposons01:24

Transposons

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Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
163
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.1K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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LTR Retrotransposons03:08

LTR Retrotransposons

17.9K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.9K
Reporter Genes02:11

Reporter Genes

11.9K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Sep 13, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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Random Insertion Reporter Gimmicks Powered by Cut-and-Paste DNA Transposons.

Yamato Kasahara1, Kentaro Semba1,2, Shinya Watanabe2

  • 1Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.

Biomedicines
|July 29, 2025
PubMed
Summary

Transposons, mobile genetic elements, can be engineered to insert DNA, creating reporter cells. Their inherent randomness enables sensitive screening for biological discovery and drug development.

Keywords:
DNA transposonrandom screeningreportertransposase

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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

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Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
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Last Updated: Sep 13, 2025

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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

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Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Transposons are mobile genetic elements that can move within genomes.
  • DNA transposons utilize a cut-and-paste mechanism for DNA integration.
  • This mechanism is being harnessed for targeted DNA insertion technologies.

Purpose of the Study:

  • To review the utility of transposon-mediated DNA integration for creating reporter cell systems.
  • To highlight how the inherent randomness of transposon integration can be leveraged for biological discovery.
  • To explore the potential of transposon-based strategies in cellular engineering and medical research.

Main Methods:

  • Review of existing literature on transposon biology and applications.
  • Discussion of strategies combining designed DNA cargo with stochastic transposon integration.
  • Exploration of the use of reporter cells for functional marker identification and pathway discovery.

Main Results:

  • Transposon-mediated integration, despite its randomness, is valuable for generating sensitive reporter cells.
  • Reporter cells facilitate efficient identification of functional markers and novel signaling pathways.
  • The stochastic nature of integration aids in establishing innovative platforms for drug screening.

Conclusions:

  • Transposon-based strategies, embracing randomness, offer powerful tools for genome-wide screening.
  • The increasing availability of transposon subfamilies enhances coverage and diversity in screening approaches.
  • This approach holds significant promise for advancing biology, cellular engineering, and medical research.