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The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 °C
Sabrina Gacem1, Eva Mocé2, Carmen Gozalbo1
1Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Valencia, Spain.
Biology
|July 29, 2025
Summary
Pyruvate and lactate improve refrigerated goat sperm quality, outperforming glucose. This research identifies optimal energetic substrates for artificial insemination, enhancing sperm preservation.
Area of Science:
- Reproductive Biology
- Animal Science
- Biochemistry
Background:
- Artificial insemination in goats requires refrigerated semen.
- The ideal energetic substrate for sperm preservation is not well-defined.
Purpose of the Study:
- To assess the impact of various energetic substrates on goat sperm metabolism and motility during refrigeration at 17 °C.
- To determine the optimal extender for maintaining sperm quality during storage.
Main Methods:
- Goat semen was diluted in PBS with glucose, fructose, pyruvate, or lactate.
- Sperm quality was evaluated using CASA for motility and flow cytometry for viability, mitochondrial membrane potential, and ROS.
- Experiments included varying substrate concentrations, NaCl, and osmolarity.
Main Results:
- Pyruvate and lactate extenders significantly enhanced sperm motility, progressivity, and viability over 48 hours at 17 °C.
- Glucose supplementation negatively impacted sperm viability and healthy population rates.
- NaCl and osmolarity adjustments did not yield significant effects on sperm parameters.
Conclusions:
- Pyruvate is a superior energetic substrate for preserving goat sperm quality and motility during refrigeration compared to glucose.
- Lactate also shows promise as an effective extender for refrigerated goat semen.
