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Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

163
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
163

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Related Experiment Video

Updated: Sep 13, 2025

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
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Effect of Pefloxacin on Clostridioides difficile R20291 Persister Cells Formation.

Camila Queraltó1,2, Iván L Calderón3, Isidora Flores2

  • 1Doctorado en Biociencias Moleculares, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.

Antibiotics (Basel, Switzerland)
|July 29, 2025
PubMed
Summary

This study identifies Clostridioides difficile persister cells, which tolerate antibiotics like pefloxacin. Understanding these persister cells is crucial for tackling antibiotic resistance and chronic infections.

Keywords:
Clostridioides difficilepefloxacinpersister cells

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Area of Science:

  • Microbiology
  • Bacteriology

Background:

  • Clostridioides difficile causes antibiotic-associated diarrhea.
  • Bacterial persisters are tolerant to antibiotics, contributing to resistance and chronic infections.
  • Pefloxacin treatment previously showed a persister-like phenotype in C. difficile.

Purpose of the Study:

  • To enrich and characterize C. difficile persister cells.
  • To understand persister cell formation during exponential growth under pefloxacin and lysis treatments.

Main Methods:

  • Enrichment of persister cells from C. difficile cultures.
  • Characterization of persister cells under pefloxacin and lysis treatments.

Main Results:

  • Identified persister cells with reduced metabolism and DNA damage.
  • Observed the involvement of toxin-antitoxin systems in persister cell generation.
  • Noted the role of Clp proteases in the formation of persister cells.

Conclusions:

  • Demonstrated the formation of C. difficile persister cells.
  • Showed that lethal concentrations of pefloxacin trigger persister cell formation.