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Comparative Analysis of Differentially Expressed Long Non-Coding RNA in Pre- and Postmenopausal Fibroids
Tsai-Der Chuang1, Shawn Rysling1, Nhu Ton1
1The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA.
International Journal of Molecular Sciences
|July 29, 2025
Summary
Menopausal status influences long non-coding RNA (lncRNA) expression in uterine fibroids. Hormone-responsive lncRNAs may be key in fibroid development and offer potential therapeutic targets.
Area of Science:
- Gynecology
- Molecular Biology
- Oncology
Background:
- Uterine fibroids (leiomyomas) are common benign tumors influenced by estrogen and progesterone.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in gene regulation and disease pathogenesis.
Purpose of the Study:
- To compare differentially expressed lncRNAs in uterine fibroids between postmenopausal and premenopausal women.
- To identify hormone-responsive lncRNAs involved in fibroid development.
Main Methods:
- RNA sequencing of fibroid and myometrium tissues from postmenopausal women.
- Comparative analysis with a premenopausal fibroid dataset.
- Quantitative PCR validation of selected lncRNAs in an expanded cohort.
Main Results:
- Significant differential expression of 3684 lncRNAs in postmenopausal fibroids.
- 741 lncRNAs showed altered expression based on menopausal status.
- Specific lncRNAs (e.g., LINC02433, LINC01449, SNHG12, H19, HOTTIP, ZEB2-AS1, CASC15, MIAT, LINC01117) exhibited distinct expression patterns related to menopausal status and MED12 mutation.
Conclusions:
- lncRNA expression profiles in uterine fibroids are modulated by menopausal status, suggesting hormonal influence.
- Identified hormone-responsive lncRNAs represent potential therapeutic targets for uterine fibroids.
