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CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: May 1, 2026

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

CRISPR/Cas12a-Based One-Tube RT-RAA Assay for PoRV Genotyping.

Mingfang Bi^1,2, Zunbao Wang¹, Kaijie Li¹

¹College of Veterinary Medicine, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, and Institute of Zoonosis, Jilin University, Changchun 130062, China.

International Journal of Molecular Sciences

|July 29, 2025

Summary

A new diagnostic tool rapidly identifies porcine rotavirus genotypes G4, G5, and G9. This advancement aids in controlling viral diarrhea in piglets and assessing vaccine effectiveness.

Keywords:

CRISPR/Cas12a PoRV RT-RAA genotyping

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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Published on: April 25, 2025

Area of Science:

Veterinary Virology
Molecular Diagnostics
Public Health Microbiology

Background:

Porcine rotavirus (PoRV) causes significant economic losses in pig farming due to viral diarrhea.
PoRV's genetic diversity and recombination potential pose public health risks through cross-species transmission.
Existing vaccine efficacy is limited by poor cross-protection between PoRV genotypes, highlighting the need for continuous surveillance.

Purpose of the Study:

To develop a rapid and efficient diagnostic platform for simultaneous genotyping of common PoRV strains.
To overcome the limitations of conventional molecular assays in terms of speed and technical requirements.
To support timely disease control and vaccine development strategies for porcine rotavirus.

Main Methods:

Development of a novel diagnostic platform integrating reverse transcription recombinase-aided amplification (RT-RAA) with CRISPR-Cas12a technology.
Utilized universal primers for simultaneous amplification of G4, G5, and G9 PoRV genotypes.
Employed sequence-specific CRISPR-Cas12a recognition for accurate and rapid genotyping.

Main Results:

The developed RT-RAA-CRISPR-Cas12a system achieved simultaneous genotyping of PoRV G4/G5/G9 within 50 minutes at 37 °C.
Demonstrated high sensitivity with a limit of detection of 10^0 copies/μL.
Clinical validation showed high concordance with established reverse transcription quantitative polymerase chain reaction (RT-qPCR) methods.

Conclusions:

The novel RT-RAA-CRISPR-Cas12a platform offers an efficient solution for rapid PoRV genotyping.
This technology can significantly aid in evaluating vaccine compatibility and optimizing epidemic control strategies.
The platform provides a valuable tool for real-time surveillance of circulating porcine rotavirus strains.