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Transcriptome Profiling Reveals Differences Between Rainbow Trout Eggs with High and Low Potential for Gynogenesis
Konrad Ocalewicz1, Artur Gurgul2, Stefan Dobosz3
1Department of Marine Biology and Biotechnology, Faculty of Oceanography and Geography, University of Gdańsk, M. Piłsudskiego 46 Av, 81-378 Gdynia, Poland.
Genes
|July 29, 2025
Summary
Maternal gene expression in rainbow trout eggs influences gynogenetic development success. Transcriptional differences correlate with doubled haploid survival, indicating early mortality causes beyond zygotic gene expression.
Area of Science:
- Aquaculture and Reproductive Biology
- Genomics and Molecular Biology
- Developmental Biology
Background:
- Gynogenetic doubled haploids (DHs) are fully homozygous individuals crucial for genetic research.
- Low survival rates in fish DHs are often attributed to recessive gene expression and gamete treatment side effects.
- Maternal gene products significantly influence early embryonic development, impacting gynogenesis efficiency.
Purpose of the Study:
- To investigate transcriptional differences in rainbow trout eggs with varying gynogenetic development potential.
- To identify maternal factors affecting the survival of gynogenetic doubled haploids (DHs).
- To utilize RNA-sequencing to understand gene expression related to egg competence for gynogenesis.
Main Methods:
- Induction of gynogenetic development in rainbow trout eggs from eight distinct females.
- Isolation and RNA sequencing (RNA-seq) of maternal RNA from eggs.
- Comparative analysis of gynogenote survival rates and egg transcriptome profiles.
Main Results:
- Significant transcriptional variations were observed between eggs from different females.
- Egg transcriptomic profiles strongly correlated with their capacity for successful gynogenesis.
- Upregulated genes in high-survival DH eggs were linked to development, cell differentiation, migration, and protein transport, including genes in oocyte maturation and early embryogenesis.
Conclusions:
- Inter-individual variation in maternal gene transcription is linked to egg competence for gynogenesis.
- A portion of rainbow trout DH mortality occurs prior to zygotic genome activation.
- Maternal effects play a critical role in the early survival of gynogenetic fish.
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