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Global Transcriptome and Weighted Gene Co-Expression Network Analyses of Cold Stress Responses in Chinese Cabbage
Jizong Zhang1, Songtao Liu2, Huibin Li1
1College of Agronomy, Hebei Agricultural University, Baoding 071000, China.
Genes
|July 29, 2025
Summary
Chinese cabbage is sensitive to cold, which can cause premature bolting. This study identified key genes and pathways involved in cold response, offering potential for improved cold stress tolerance in breeding.
Area of Science:
- Plant Science
- Molecular Biology
- Genomics
Background:
- Chinese cabbage (Brassica rapa ssp. Pekinensis) growth is sensitive to cold temperatures, leading to premature bolting and reduced yield.
- Understanding the genetic and molecular mechanisms of cold response is crucial for improving crop quality and yield.
Purpose of the Study:
- To explore key genes and metabolic pathways regulating Chinese cabbage cold response.
- To identify candidate genes and co-expression modules associated with cold stress tolerance.
Main Methods:
- Comparative leaf RNA-seq transcriptome analysis at different temperatures (6, 9, 12, 15 °C).
- KEGG enrichment analysis, K-means clustering, and Weighted Gene Co-expression Network Analysis (WGCNA).
- Phenotypic and physiological measurements under cold stress conditions.
Main Results:
- Identified 1832 differentially expressed genes (DEGs) in response to cold stress.
- Cold stress significantly affected sulfur metabolism, secondary metabolites biosynthesis, and photosynthesis pathways.
- Identified key co-expression modules (M1, M2, M3, M6) and candidate hub genes (MYB, bZIP, WRKY transcription factors) linked to cold response and vernalization.
Conclusions:
- The study provides a set of candidate cold-stress-responsive genes and co-expression modules.
- These findings may aid in breeding Chinese cabbage varieties with enhanced cold stress tolerance.
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