Some problems involved in employing the ERG c-wave in pharmacological experiments: conditioning in pigmented rabbits

The large inter- and intra-individual variations in ERG c-waves have prevented the application of the component in pharmacological experiments on the retinal pigment epithelial function. We examined some of the problems involved in employing the c-wave in pharmacological experiments using rabbit retinas. 1) Under our experimental conditions, the c-wave amplitudes in some rabbits reached equilibrium under intermittent stimuli, while in others they increased with time and did not reach a constant level in 3 h. 2) The c-wave amplitudes of the right and left eyes were similar, indicating that the contralateral eye can be used as a control during local administration of drugs. 3) The c-wave amplitude under 50 lux light had smaller inter-individual variations than that in the dark. This may be a useful indicator of the retinal pigment epithelium function when drugs are administered systemically.