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Hepatoma-derived growth factor and non-coding RNA network in ovarian cancer patients

Reza Ganjali1, Setare Nassiri2, Narges Zamani3

  • 1Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Biochemistry and Biophysics Reports
|July 29, 2025
PubMed
Abstract

Insights

Ovarian cancer (OC) progression is linked to the LINC00839-miR-345-5p-HDGF axis. This study identifies Hepatoma-derived growth factor (HDGF) as a potential biomarker for OC and suggests targeting lncRNAs and miRNAs for new therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ovarian cancer (OC) is a lethal gynecologic malignancy with limited diagnostic biomarkers.
  • Hepatoma-derived growth factor (HDGF) is an oncogene implicated in tumor progression, but its role in OC is underexplored.
  • Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) may regulate oncogenic pathways via competing endogenous RNA (ceRNA) networks.

Purpose of the Study:

  • To investigate the regulatory role of the lncRNA LINC00839, miR-345-5p, and HDGF in ovarian cancer.
  • To explore the potential of the LINC00839-miR-345-5p-HDGF axis in OC pathogenesis.
  • To evaluate HDGF as a potential diagnostic biomarker for ovarian cancer.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) was used to analyze HDGF, LINC00839, and miR-345-5p expression in 50 OC and 50 normal ovarian tissues.
  • Bioinformatic tools were utilized to predict RNA-RNA interactions and construct a ceRNA network.
  • Statistical analyses, including correlation analysis and receiver operating characteristic (ROC) curve analysis, were performed.

Main Results:

  • HDGF and LINC00839 were significantly upregulated, while miR-345-5p was downregulated in OC tissues (p < 0.001).
  • HDGF expression positively correlated with LINC00839 and inversely with miR-345-5p, indicating a ceRNA regulatory axis.
  • Elevated HDGF levels were associated with advanced tumor stage, larger tumor size, and metastasis. HDGF serum levels showed promising diagnostic potential (AUC = 0.88).

Conclusions:

  • The LINC00839-miR-345-5p-HDGF axis is a key regulatory network in ovarian cancer.
  • HDGF may serve as a clinically relevant biomarker for OC progression.
  • LINC00839 and miR-345-5p represent potential therapeutic targets for ceRNA-based interventions in OC.
Keywords:
Hepatoma-derived growth factorLINC00839Ovarian cancermiR-345-5pmiR-384

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