Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fermentation01:29

Fermentation

97.1K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
97.1K
Microbial Fermentation01:23

Microbial Fermentation

1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Microbes in Food Production01:29

Microbes in Food Production

403
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
403
Microbes in Beverage Production01:25

Microbes in Beverage Production

298
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
298
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spin Waves Excited by Hard X-Ray Transient Gratings.

Physical review letters·2026
Same author

Statistical optimization of acid phosphatase production by <i>Trichoderma</i> spp. in submerged fermentation.

3 Biotech·2026
Same author

Optimized GA<sub>3</sub> Production by <i>Aspergillus niger</i> and <i>Fusarium fujikuroi</i>: From Low-Cost Fermentation to Agronomic Application.

ACS omega·2026
Same author

Electronic Structure of Coenzyme B<sub>12</sub> Excited States as Probed by Time-Resolved X-ray Absorption and Emission Spectroscopies.

The journal of physical chemistry. B·2025
Same author

Insights into using <i>Kosakonia oryzae</i> AG15 in liquid formulations containing sodium alginate, glycerol, and gum arabic for the sustainable development of <i>Phaseolus vulgaris</i> L.

3 Biotech·2025
Same author

Application of Ion-Exchange Membranes With Graphene Oxide in the Removal of Metal Ions Using the Electrodialysis Process.

Water environment research : a research publication of the Water Environment Federation·2025

Related Experiment Video

Updated: Apr 30, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.1K

Acid Phosphatase Produced by Trichoderma harzianum in Solid Fermentation Using Millet.

Jussara Maria Martins de Almeida Afonso1, Frederico Alves Lima1, Miriam Maria de Resende1

  • 1Chemical Engineering Faculty, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Campus Santa Mônica, Bloco 1K, 38408-144 Uberlândia, MG, Brazil.

ACS Omega
|July 29, 2025
PubMed
Summary
This summary is machine-generated.

This study optimized the production of acid phosphatases (AcPases) using solid-state fermentation with millet. The best conditions yielded significant enzyme activity and biomass, crucial for organic phosphorus utilization.

More Related Videos

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

15.8K
Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

380

Related Experiment Videos

Last Updated: Apr 30, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.1K
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

15.8K
Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

380

Area of Science:

  • Biochemistry
  • Enzymology
  • Microbial Biotechnology

Background:

  • Acid phosphatases (AcPases) are crucial enzymes for organic phosphorus absorption and utilization.
  • Fungi play a key role in converting insoluble phosphorus into soluble forms via enzymatic hydrolysis.
  • AcPases function optimally at acidic pH (below 6.00).

Purpose of the Study:

  • To evaluate and optimize the production and extraction of acid phosphatases.
  • To investigate the potential of solid-state fermentation (SSF) using millet as a substrate.
  • To determine optimal conditions for enzyme activity and biomass production.

Main Methods:

  • Solid-state fermentation (SSF) using millet as a substrate.
  • Preliminary screening to identify optimal substrate and strain potential.
  • Central Composite Design (CCD) to optimize variables: millet mass, inoculum concentration, and moisture.
  • Extraction using Tween 80.

Main Results:

  • Millet was identified as the most effective substrate for enzyme production.
  • Optimized SSF conditions using CCD yielded significant biomass (9.27 ± 0.53 g/L) and acid phosphatase activity (36.09 ± 0.61 U/mL).
  • Cultivation in sterile distilled water and extraction with Tween 80 enhanced results.

Conclusions:

  • Solid-state fermentation with millet is an effective method for producing acid phosphatases.
  • Optimized conditions significantly increased enzyme yield and biomass.
  • This process holds potential for efficient organic phosphorus management.