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Updated: Sep 13, 2025
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Isolation of Elasnin and Antimycin Derivatives from Syo_1.56 SARP-Expressing Streptomyces sp. RK18-A0406 and Their
Islam A Abdelhakim1,2, Yushi Futamura3, Toshihiko Nogawa4
1Natural Product Biosynthesis Research Unit, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan.
Abstract:
Exploration of pathway-specific regulators is an important strategy to uncover hidden natural products. Recently, we successfully enhanced the production of novel elasnins by expressing Syo_1.56 Streptomyces antibiotic regulatory protein (SARP) in Streptomyces sp. RK18-A0406. However, initial investigations yielded a limited number of novel elasnin derivatives. To expand the chemical repertoire of metabolites produced by this engineered strain, we scaled up fermentation and implemented targeted metabolite profiling in this study, resulting in the isolation of six new elasnin C derivatives (1-6) and three new antimycins (7-9). The identified compounds exhibited antibacterial activity (IC50 = 0.6-2.6 μg/mL) and antimalarial potential (IC50 = 1.9-3.9 μg/mL). These findings underscore the effectiveness of regulator-based activation in unlocking the chemical diversity of microbial natural products.
