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Agricultural, Veterinary And Food Sciences
Agricultural Biotechnology
Agricultural Biotechnology Diagnostics (incl. Biosensors)
An Optimized Spe-uplc-ms/ms Method For Simultaneous Quantification Of 11 Tetracyclines In Dairy Products.

Home
Research Domains
Agricultural, Veterinary And Food Sciences
Agricultural Biotechnology
Agricultural Biotechnology Diagnostics (incl. Biosensors)
An Optimized Spe-uplc-ms/ms Method For Simultaneous Quantification Of 11 Tetracyclines In Dairy Products.

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An optimized SPE-UPLC-MS/MS method for simultaneous quantification of 11 tetracyclines in dairy products.

Pengfei Gao^1,2, Boxing Yin³, Renqin Yang³

¹College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Journal of the Science of Food and Agriculture

|July 29, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new method rapidly detects 11 tetracyclines (TCs) in dairy products using UPLC-MS/MS. This advancement enhances food safety monitoring and ensures compliance with international antibiotic residue limits.

Keywords:

UPLC–MS/MS milk safety multi‐residue analysis tetracycline antibiotics

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Area of Science:

Analytical Chemistry
Food Science
Pharmacology

Background:

Rising antibiotic use in animal husbandry increases tetracycline (TC) residues in food.
Current detection methods for TCs in dairy lack efficiency and multi-residue capability.
Urgent need for rapid, precise, and cost-effective TC monitoring in dairy products.

Purpose of the Study:

To develop and validate a streamlined method for simultaneous detection of multiple tetracyclines in dairy products.
To ensure the method meets international maximum residue limits for food safety.

Main Methods:

Integrated a three-step purification process (PRiME HLB cartridges) with UPLC-MS/MS.
Utilized Na2EDTA-McIlvaine buffer for optimized extraction.
Employed a BEH C18 column for rapid chromatographic separation.

veterinary drug residues

Main Results:

Achieved high recovery rates (83.11-107.61%) with low relative standard deviation (<5.43%).
Enabled simultaneous detection of 11 TCs (parent compounds and metabolites) within 9 minutes.
Demonstrated exceptional sensitivity with low limits of detection and quantification (0.02-0.83 μg/kg and 0.07-2.78 μg/kg).
Validated intra-/inter-day precision (RSD 3.03-9.33%) in real milk and milk powder samples.

Conclusions:

The developed method offers rapid sample preparation and simultaneous UPLC-MS/MS quantification of 11 TCs in dairy.
High accuracy, sensitivity, and regulatory compliance make it vital for food safety monitoring.
Addresses antibiotic residue risks and supports international trade harmonization.