Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

2.7K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
2.7K
The Retina01:32

The Retina

70.6K
The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
70.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Type 2 and type 1 diabetes have opposing effects on the systemic murine complement alternative pathway.

iScience·2026
Same author

Advances in analytical methods for regulatory complement proteins in research and clinical applications.

Talanta·2026
Same author

Engineering Liposomes and Polymer Conjugates: A Platform for Mechanistic Complement Activation Studies and Controlled Release Applications.

ACS applied materials & interfaces·2026
Same author

Complement Activation as a Predictor of Postoperative Delirium in Elderly Spine Surgery Patients.

International journal of molecular sciences·2026
Same author

Beta-cell-specific C3 deficiency exacerbates metabolic dysregulation and insulin resistance in obesity.

Molecular metabolism·2025
Same author

Development of a Novel Homogeneous Liposome-Based One-Step Assay for SARS-CoV-2 Antibody Detection in Human Serum Based on Fluorescent Liposomes and Complement Activity.

Analytical chemistry·2025

Related Experiment Video

Updated: Sep 13, 2025

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
08:33

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Published on: February 10, 2021

6.4K

Complement C3 Activation in the Human Retinal Pigment Epithelium.

Juliane Schikora1, Antonia Nickel1, Jasmin Bergert1

  • 1Experimental Ophthalmology, Philipps-Universität Marburg, Marburg, Germany.

Investigative Ophthalmology & Visual Science
|July 29, 2025
PubMed
Summary

High maturation conditions improve retinal pigment epithelial (RPE) cell models, enhancing their features and complement biology. High maturation induced pluripotent stem cell-derived RPE (iPSC-RPE) cells closely mimic native RPE for robust in vitro studies.

More Related Videos

Direct-Coupled Electroretinogram DC-ERG for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
09:10

Direct-Coupled Electroretinogram DC-ERG for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Published on: July 14, 2020

5.4K
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
08:28

Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology

Published on: November 6, 2010

19.4K

Related Experiment Videos

Last Updated: Sep 13, 2025

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
08:33

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Published on: February 10, 2021

6.4K
Direct-Coupled Electroretinogram DC-ERG for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
09:10

Direct-Coupled Electroretinogram DC-ERG for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Published on: July 14, 2020

5.4K
Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
08:28

Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology

Published on: November 6, 2010

19.4K

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Immunology

Background:

  • Epithelial maturation is critical for the functionality of retinal pigment epithelial (RPE) cell models.
  • Understanding how maturation conditions affect RPE characteristics and complement biology is essential for accurate in vitro studies.

Purpose of the Study:

  • To investigate the impact of different maturation conditions on RPE models.
  • To compare ARPE-19 and induced pluripotent stem cell-derived RPE (iPSC-RPE) cells under varying maturation states.
  • To analyze RPE complement biology and C3 processing in response to maturation.

Main Methods:

  • ARPE-19 and iPSC-RPE cells were cultured under low (LM) and high (HM) maturation conditions.
  • Phenotypic, marker expression, and functional properties were assessed.
  • Complement component expression (transcript, protein, secretion) and intracellular C3 processing were analyzed.

Main Results:

  • High maturation (HM) enhanced epithelial features in both RPE models.
  • HM iPSC-RPE cells showed improved polarity, apical marker localization, and transepithelial resistance.
  • Maturation influenced complement component expression and secretion, with HM iPSC-RPE showing increased C3a, C3(H2O), and iC3b secretion and distinct C3 localization.

Conclusions:

  • High maturation iPSC-RPE cells represent a robust in vitro model closely mimicking native RPE.
  • RPE cells autonomously produce complement components with maturation-dependent C3 activation profiles.
  • This study provides a basis for investigating C3 functionality in RPE homeostasis and retinal diseases.