Related Experiment Video

Updated: Jul 30, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
11:25

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Published on: October 4, 2017

6.7K

Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli

Sven O Dahms1, Alexander C Wieland1, Hans Brandstetter1

  • 1University of Salzburg, Department of Biosciences and Medical Biology, Hellbrunner Strasse 34, 5020 Salzburg, Austria; Center of Tumor Biology and Immunology, University of Salzburg, 5020 Salzburg, Austria.

STAR Protocols
|July 29, 2025
PubMed

Keywords:
Biotechnology and bioengineeringProtein BiochemistryProtein expression and purification

More Related Videos

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

18.2K
Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate06:03

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

Published on: October 29, 2021

4.5K

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...