MMP-9 Activation via ROS/NF-κB Signaling in Colorectal Cancer Progression: Molecular Insights and...

Area of Science:

• Oncology
• Biochemistry
• Molecular Biology

Background:

• Colorectal cancer (CRC) involves complex interactions between inflammation, oxidative stress, and extracellular matrix remodeling.
• The reactive oxygen species (ROS)-nuclear factor kappa B (NF-κB)-matrix metalloproteinase-9 (MMP-9) axis is significant in CRC invasion and metastasis.
• Understanding this axis is crucial for developing targeted therapies.

Purpose of the Study:

• To analyze advanced oxidation protein products (AOPPs), NF-κB expression, and MMP-9 activity in CRC tissues.
• To investigate the correlation between oxidative stress markers and MMP-9 activity.
• To evaluate MMP-9 as a potential diagnostic and theranostic marker for CRC.

Main Methods:

• Analysis of AOPPs, NF-κB, and MMP-9 in tumor, adjacent, and healthy colon tissues from 50 CRC patients.
• Utilized ELISA, spectrophotometry, and indirect immunofluorescence for biochemical analyses.
• Correlated marker levels with clinical data and disease progression.

Main Results:

• Significantly elevated AOPPs, NF-κB, and MMP-9 in tumor tissues compared to controls.
• Adjacent tissues showed increased NF-κB and MMP-9 compared to healthy tissues.
• AOPP levels strongly correlated with MMP-9 activity; MMP-9 had the highest predictive value for CRC.

Conclusions:

• The ROS-NF-κB-MMP-9 axis is integral to colorectal cancer progression, particularly in T2-T3 stages.
• MMP-9 emerges as a promising diagnostic and theranostic marker for CRC.
• Targeting this pathway offers potential therapeutic strategies for CRC invasion and recurrence.