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Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Related Experiment Video

Updated: Sep 13, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Assessing the Methylation Status of Two Potential Key Factors Involved in Cervical Oncogenesis.

Alina Fudulu¹, Marinela Bostan¹, Iulia Virginia Iancu¹

¹Stefan S. Nicolau Institute of Virology, Romanian Academy, 030304 Bucharest, Romania.

|July 29, 2025

Summary

Reduced promoter methylation of EIF4G3 and SF3B1 genes may indicate abnormal cervical cytology. This finding could aid in early detection of cervical cancer, complementing existing screening methods like Pap smears.

Keywords:

DNA methylation HPV infection cervical cancer qRT-PCR

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Cervical cancer, primarily caused by high-risk Human Papillomavirus (hrHPV), presents a substantial global health challenge.
While Pap smears are effective for early detection, novel biomarkers are needed for risk stratification.
DNA methylation is an area of active research for identifying such biomarkers.

Purpose of the Study:

To investigate the expression levels of EIF4G3 and SF3B1 genes in cervical precursor lesions and tumors.
To determine the methylation status of the promoters of EIF4G3 and SF3B1.
To explore the correlation between gene expression and promoter methylation in cervical tissues.

Main Methods:

Cross-sectional study design.
Quantitative reverse transcription PCR (qRT-PCR) for gene expression analysis.
Bisulfite conversion followed by methylation analysis to assess promoter methylation status.

Main Results:

Both EIF4G3 and SF3B1 exhibited highest mRNA expression in squamous cell carcinoma (SCC) samples.
Increased promoter methylation percentages were observed in the control group compared to tumor groups.
A significant inverse correlation was found between gene expression levels and promoter methylation for both EIF4G3 and SF3B1.

Conclusions:

A decreasing trend in promoter methylation from normal (NILM) to SCC was observed for both genes.
Reduced promoter methylation of EIF4G3 and SF3B1 may serve as a potential biomarker for abnormal cervical cytology.
These findings contribute to the ongoing search for molecular markers in cervical cancer diagnostics.