Discordant effects of maternal age on the human MII oocyte transcriptome
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View abstract on PubMed
Summary
This summary is machine-generated.Advanced maternal age impacts oocyte gene expression, but signatures are inconsistent. A meta-analysis identified 25 age-correlated genes, including proteasomal subunits, suggesting multifactorial oocyte aging with limited transcriptomic roles.
Area Of Science
- Reproductive Biology
- Genomics
- Aging Research
Background
- Advanced maternal age is linked to reduced oocyte quality.
- Existing research shows age-related changes in oocyte gene expression, but with limited consensus on specific genes.
- Understanding these transcriptomic changes is crucial for reproductive health.
Purpose Of The Study
- To characterize the effects of age on the human MII oocyte transcriptome.
- To identify reproducible age-associated gene expression signatures.
- To explore the role of transcriptomic changes in oocyte aging.
Main Methods
- Generated single-oocyte Smart-seq2 data from younger and older donors.
- Cross-referenced findings with 12 previous studies (human and mouse).
- Implemented a meta-analytic approach combining new and existing oocyte transcriptomic data (150 libraries total).
Main Results
- Considerable discordance found between datasets, indicating poor reproducibility of age-related gene expression signatures.
- Identified 40 genes with age-altered expression across multiple studies, including those involved in proteostasis.
- Meta-analysis revealed 25 genes significantly correlated with age, with 14 validated by RT-PCR, including downregulated proteasomal subunits (PSMA1, PSMA2) in older oocytes.
Conclusions
- Oocyte aging is a multifactorial process where transcriptomic changes play a restricted role.
- Pronounced inter-oocyte heterogeneity in transcription exists.
- Proteomic changes may have more significant roles in oocyte aging than previously thought.
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