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Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

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Related Experiment Video

Updated: May 6, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

High-Throughput Pipeline for Protein Expression and Solubility Profiling Using Synthetically Generated Plasmids.

Supratim Dey¹, Saurabh Gireesan¹, Theodora Solovou¹

¹Department of Microbiology-Immunology and Center for Structural Biology of Infectious Diseases, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.

Current Protocols

|July 29, 2025

Summary

This study presents a high-throughput pipeline for protein expression and purification, leveraging genomics data. The method efficiently screens soluble protein targets for structural and functional genomics projects.

Keywords:

96‐well HTP expression and solubility high‐throughput transformation

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Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

Genomics and metagenomics provide vast gene resources for protein studies.
Efficient protein expression and purification are crucial for structural and functional genomics.
Current methods may not be optimized for large-scale, time-efficient screening.

Purpose of the Study:

To outline a comprehensive pipeline for high-throughput protein expression and purification.
To enable efficient screening of a large repertoire of protein targets.
To facilitate structural and functional genomics research.

Main Methods:

Strategic selection of protein targets for commercial synthetic gene services.
High-throughput transformation, expression, and solubility screening in a 96-well plate format.
Optimization of protocols for time-efficient protein production.

Main Results:

A validated pipeline for large-scale protein target selection and screening.
Demonstrated feasibility of high-throughput protein expression and solubility determination.
Successful identification of soluble proteins suitable for purification.

Conclusions:

The developed pipeline significantly enhances the efficiency of producing soluble proteins for research.
This approach is broadly applicable to structural and functional genomics and other large-scale screening projects.
The method provides a time-efficient solution for protein purification needs.