Hyaluronan-Based Rapid-Gelling Hemostatic Powder to Bridge Dynamic Wound Sealing and Tissue Regeneration

Hemostatic materials combining rapid bleeding control with regenerative potential remain a major clinical need. Here, we report a bioinspired hemostatic powder (GSHD) engineered through molecular conjugation of thiolated gelatin and dopamine-modified hyaluronic acid. Upon contact with biofluids, GSHD rapidly gels via dynamic thiol-catechol cross-linking, achieving ultrafast hydration (<5 s) and forming a robust hydrogel. This hydrogel exhibits high blood absorption (569.2 ± 46.3%), strong tissue adhesion (28.3 ± 3 kPa), and burst pressure resistance (216.2 ± 10.5 mmHg), enabling effective sealing under high-flow conditions. In vivo tests in rat liver, cardiac, and femoral artery models show rapid hemostasis (<30 s) with minimal blood loss. Additionally, GSHD promotes M2 macrophage polarization, enhances angiogenesis, and supports organized collagen deposition, facilitating tissue regeneration. By integrating rapid sealing, antioxidant activity, and biomimetic extracellular matrix cues, GSHD represents a paradigm shift in hemostatic design, bridging emergency care with functional tissue repair.