Proteomics Insights Into Gingival Aging: The Role of Oxidative Stress and Key Signaling Pathways
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View abstract on PubMed
Summary
This summary is machine-generated.Oxidative stress accelerates gingival aging by disrupting epidermal growth factor receptor (EGFR) signaling and increasing cytochrome P450 (CYP450) activity. Targeting these pathways may offer anti-aging strategies for gums.
Area Of Science
- Oral biology
- Aging research
- Molecular mechanisms of aging
Background
- Gingival aging is linked to oxidative stress, but molecular details are unclear.
- Understanding these mechanisms is crucial for developing anti-aging interventions.
Purpose Of The Study
- To investigate the role of oxidative stress in gingival aging using proteomic and functional analyses.
- To elucidate the molecular pathways involved in age-related changes in gingival tissue.
Main Methods
- Proteomic analysis of young vs. aged mouse gingival tissues using 4D label-free mass spectrometry.
- Bioinformatics analyses (GO, KEGG, GSEA, PPI) of differentially expressed proteins.
- In vitro aging model using human periodontal ligament fibroblasts stimulated with H2O2, alongside molecular and biochemical assays.
Main Results
- Aged gingiva showed increased reactive oxygen species (ROS), decreased epidermal growth factor receptor (EGFR), and inhibited PI3K-AKT signaling.
- Proteomics revealed 224 differentially expressed proteins; upregulated proteins were redox-related, downregulated ones involved cell proliferation and repair.
- Inhibiting cytochrome P450 (CYP450) reduced ROS and aging phenotypes; EGFR inhibition worsened fibroblast function, partially reversed by antioxidant pretreatment.
Conclusions
- Oxidative stress drives gingival aging and functional decline by impairing EGFR signaling and enhancing CYP450 activity.
- This study offers new insights into gingival aging mechanisms.
- Targeting oxidative stress and EGFR signaling presents potential anti-aging therapeutic strategies for gingival tissues.
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