1,2,3,6-Tetra-O-Galloyl-β-D-Glucopyranose Induces Apoptosis and Ferroptosis in Colon Cancer Cells by Inhibiting the

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Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
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Suhyeon Kim1, MinKyun Na2, Sangtaek Oh1

  • 1Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea.

Journal of Microbiology and Biotechnology
|July 29, 2025
PubMed

Insights

1,2,3,6-Tetra-O-galloyl-β-D-glucopyranose (TAGP) from Trapa japonica inhibits colon cancer growth by promoting β-catenin degradation. TAGP also induces apoptosis and ferroptosis, showing potential as a novel colon cancer therapeutic.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Canonical Wnt pathway signaling is frequently dysregulated in colon cancer, leading to β-catenin stabilization.
  • Targeting molecular aberrations in this pathway presents a therapeutic opportunity for colon cancer treatment.

Purpose of the Study:

  • To identify novel inhibitors of the canonical Wnt signaling pathway.
  • To investigate the anti-cancer effects of 1,2,3,6-Tetra-O-galloyl-β-D-glucopyranose (TAGP) in colon cancer cells.

Main Methods:

  • TAGP was isolated from Trapa japonica.
  • Effects of TAGP on β-catenin phosphorylation and degradation were assessed.
  • Cell proliferation, apoptosis, and ferroptosis markers were analyzed in colon cancer cells treated with TAGP.

Main Results:

  • TAGP inhibited canonical Wnt signaling by promoting β-catenin phosphorylation and subsequent degradation.
  • TAGP reduced levels of Wnt/β-catenin target genes, Cyclin D1 and c-Myc.
  • TAGP exhibited antiproliferative activity, induced apoptosis (caspase activation, Annexin-V positivity), and promoted ferroptosis (lipid peroxidation, Fe2+ accumulation) in colon cancer cells.

Conclusions:

  • TAGP enhances β-catenin turnover, thereby inhibiting colon cancer cell proliferation.
  • TAGP induces both apoptosis and ferroptosis, suggesting a multi-targeted therapeutic mechanism.
  • TAGP demonstrates significant potential as a chemotherapeutic agent for human colon cancer.
Keywords:
1,2,3,6-tetra-O-galloyl-β-D-glucopyranosecolon cancerdegradationphosphorylationβ-Catenin

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