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Updated: May 11, 2026
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Complete genome analysis of a novel phenui-like virus in Morus alba
Hanze Zheng1, Yangwei Gao1, Xiaodi Hu1
1State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
Abstract:
Mulberries (genus Morus) are important to sericulture. Due to interspecific hybridization and the existence of various cultivars, the virome of many Morus species is still unknown. In this study, we identified a novel negative-sense phenui-like virus in Morus alba and determined its genome sequence. The virus possesses three linear single-stranded negative-sense RNA segments: RNA1 (7,329 nt), RNA2 (1,276 nt), and RNA3 (1,222 nt). Each of the three segments contains an open reading frame (ORF), encoding the RNA-dependent RNA polymerase (RdRP), the movement protein (MP), and the nucleocapsid protein (N), respectively. Phylogenetic analysis placed this virus within a clade including members of the genus Rubodvirus (family Phenuiviridae, order Hareavirales, class Bunyaviricetes) with raspberry rubodvirus 1, grapevine Muscat rose virus, and apple rubbery wood virus 2 being the closest relatives. Small interfering RNA (siRNA) sequencing data suggested that the virus replicates in Morus alba. Our study provides the first report of a rubodvirus in Morus alba.
